Purification and Characterization of D-psicose 3 Epimerase (DPEase) From Escherichia coli BL21 (DE3) pET21b dpe
نویسندگان
چکیده
منابع مشابه
Genomic and transcriptomic landscape of Escherichia coli BL21(DE3)
Escherichia coli BL21(DE3) has long served as a model organism for scientific research, as well as a workhorse for biotechnology. Here we present the most current genome annotation of E. coli BL21(DE3) based on the transcriptome structure of the strain that was determined for the first time. The genome was annotated using multiple automated pipelines and compared to the current genome annotatio...
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Two mutant strains of Escherichia coli BL21(DE3), called C41(DE3) and C43(DE3) and originally described by Miroux and Walker, are frequently used to overcome the toxicity associated with overexpressing recombinant proteins using the bacteriophage T7 RNA polymerase expression system. Even when the toxicity of the plasmids is so high that it prevents transformation in the strain BL21(DE3), the to...
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Diaminopimelic acid epimerase was purified from Escherichia coli. The enzyme is a monomer of Mr = 34,000. Diaminopimelic acid epimerase is not a pyridoxal phosphate-dependent enzyme: there is no evidence for pyridoxal phosphate in the ultraviolet spectrum of the purified enzyme, and the epimerase is not inactivated by carbonyl reagents such as hydroxylamine and sodium borohydride. Exchange of t...
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Escherichia coli BLR(DE3) is a commercially available recA-deficient derivative of BL21(DE3), one of the most widely used strains for recombinant protein expression. Here, we present the full-genome sequence of BLR(DE3) and highlight additional differences with its parent strain BL21(DE3) which were previously unreported but may affect its physiology.
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2020
ISSN: 1028-8880
DOI: 10.3923/pjbs.2020.561.566